Anti-Neutrophil Cytoplasmic Antibodies, Serum - Saskatoon
Discipline
Immunodiagnostics
Overview
Description
Test Ordering Requirements
Forms Required
Alias
- ANCA
- Myeloperoxidase Antibodies (MPO)
- Proteinase 3 Antibodies (PR3)
Specimen Information
Specimen types accepted
- Serum
Specimen collection container
- Preferred Collection Container: Gold (gel)
- Alternative Collection Container: Red
Required volume
- Optimal Volume: 5.0 ml Full collection tube
- Minimum Volume: 2.0 ml
(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
Transport and stability
- Serum must be removed from cells within 4 hours of collection
- Serum must be removed from gel as soon as possible after receipt in the laboratory
- Stable up to 7 days at 2-8°C
Rejection criteria
- Samples held at ambient temperature for >48 hours
- Specimens violating the proper collection/storage protocols
- Pathology and Laboratory Medicine Acceptance and Rejection Criteria
Performance
Methodology
- Multiplex Bead Immunoassay
Days/times performed
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Maximum laboratory time
Results reporting
- 2-7 days
Reflex testing
- Confirmatory IFA performed on positive results
Specimen retention time
- 2 week
Last Updated: January 15, 2026