Anti-Neutrophil Cytoplasmic Antibodies, Serum - Saskatoon

Overview

Description

Test ordering requirements

Forms required

• Community Laboratory Requisition Form #101064

Alias

• ANCA
• Myeloperoxidase Antibodies (MPO)
• Proteinase 3 Antibodies (PR3)

Specimen Information

Specimen types accepted

• Serum

Specimen collection container

• Preferred Collection Container: Gold (gel)
• Alternative Collection Container: Red

Required volume

• Optimal Volume: 5.0 mL Full collection tube
• Minimum Volume: 2.0 mL

(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)

Transport and stability

• Serum must be removed from cells within 4 hours of collection
• Serum must be removed from gel as soon as possible after receipt in the laboratory
• Stable up to 7 days at 2-8°C

Rejection criteria

• Samples held at ambient temperature for >48 hours
• Specimens violating the proper collection/storage protocols
• Pathology and Laboratory Medicine Acceptance and Rejection Criteria

Performance

Methodology

• BioPlex 2200 multiplex immunoassay

Days/times performed

Availability	Monday-Friday, except statutory holidays (8:00-16:00) Urgent testing is available upon approval by the Biochemist on Call, please contact through switchboard at 306-655-5000.
Testing site	Immunodiagnostics Laboratory, SPH

 

Maximum laboratory time

Results reporting

• 7 days

Reflex testing

• Confirmatory IFA performed on positive results

Specimen retention time

• 21 days

Last Updated: October 13, 2026